THE PREPARATION OF DIPHOSPHOPYRIDINE NUCLEOTIDE WITH ION EXCHANGE RESIN
نویسندگان
چکیده
منابع مشابه
The preparation of diphosphopyridine nucleotide with ion exchange resin.
The older methods for the preparation of diphosphopyridine nucleotide (DPN) were reviewed in 1942 by Schlenk (1). In 1947 Sumner et al. (2) outlined the need for a greatly simplified method for the preparation of this coenzyme. These authors described a procedure which did away with heavy metal fractionation. Recently, Clark et al. (3) made several modifications in the above method. The LePage ...
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Estimation of plasma catecholamine concentrations by "high-performance" liquid chromatography, although increasingly used, still presents difficulties. We describe a method for clean-up of plasma samples on cation-exchange resin before they are extracted with alumina. This procedure eliminates many of the interfering compounds often present in plasma extracts prepared with the alumina treatment...
متن کاملThe agglutination of ion exchange resin particles coated with polysaccharide.
NH20H + DPN2H -* NH40H + DPN. If NH4OH is used as substrate and the pH maintained above neutrality, the reverse reaction can be demonstrated (figure 1). In the absence of substrate, extract, or coenzyme no decrease in transmission was noted. NH4C1 and (NH4)2S04 were inactive as substrates at pH 7.0. The enzyme is stable at 60 C for 5 minutes, but not at 65 C. No loss of activity was noted after...
متن کاملInfluence of metallic chelates on the diphosphopyridine nucleotide oxidase and diphosphopyridine nucleotide-cytochrome c reductase systems.
The need for additional study on the specific relation between metallic ions and metabolic function, especially in the case of the diphosphopyridine nucleotide oxidase system, has been expressed by Mahler (1) and others. This is particularly indicated in view of the fact that a number of the known components of this system contain iron. While it is broadly concluded that iron compounds facilita...
متن کاملEnzymatic studies with analogues of diphosphopyridine nucleotide.
It has been previously shown (l-3) that the nicotinamide moiety of diphosphopyridine nucleotide can be replaced by other pyridine bases without the loss of the coenzyme function. The observation that both the 3-acetylpyridine and pyridine-3aldehyde analogues of diphosphopyridine nucleotide function as coensymes with various dehydrogenases (3) indicated that the amide group on DPN was not essent...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56171-8